Hotspot

trnH-GUG-psbA

LSC · 85-388 · 304 bp

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Pi 0.0338
MarkerSeek score 69.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
304
variable_sites
18
pi
0.0338
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 370-392 bp 1.0 86.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 384-406 bp 1.0 85.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 383-405 bp 1.0 85.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 385-407 bp 1.0 85.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 422-444 bp 1.0 84.9