Hotspot

ORF192

LSC · 11751-12329 · 579 bp

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Pi 0.0725
MarkerSeek score 75.00
Primer available yes
Label ORF192

Feature Metadata

label_name
ORF192
feature_type
gene
region
LSC
length_bp
579
variable_sites
42
pi
0.0725
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF192_p1 AGCGAACATCAATTGCAACG GTCCCGGTAATCGAAGGTTC 45.0 55.0 58.0 58.1 1064-1134 bp 1.0 89.3
ORF192_p2 GCGAACATCAATTGCAACGA GTCCCGGTAATCGAAGGTTC 45.0 55.0 58.0 58.1 1063-1133 bp 1.0 89.3
ORF192_p3 AGCGAACATCAATTGCAACG AAGGATTTGGGCAGGTCTTG 45.0 50.0 58.0 58.1 1256-1266 bp 1.0 88.7
ORF192_p4 AGCGAACATCAATTGCAACG GCAGGTCTTGACACCAAAGA 45.0 50.0 58.0 58.0 1246 bp 0.5 59.5
ORF192_p5 GCGAACATCAATTGCAACGA GCAGGTCTTGACACCAAAGA 45.0 50.0 58.0 58.0 1245 bp 0.5 59.4