Hotspot

rpl32-ndhF

SSC · 124241-125360 · 1120 bp

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Pi 0.051
MarkerSeek score 75.00
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1120
variable_sites
57
pi
0.051
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 GAGCCAATTTTCGCGTCAAA TACATGAAGGGGGAATTGCG 45.0 50.0 58.0 57.9 2073-2092 bp 1.0 82.4
rpl32-ndhF_p2 GAGCCAATTTTCGCGTCAAA AACCAAAATCCCCAACACGA 45.0 45.0 58.0 57.9 1894-1913 bp 1.0 82.3
rpl32-ndhF_p3 GAGCCAATTTTCGCGTCAAA TGTCGCTGACGGAAAAAGAA 45.0 45.0 58.0 58.1 1428 bp 0.5 59.2
rpl32-ndhF_p4 GAGCCAATTTTCGCGTCAAA TTGTCGCTGACGGAAAAAGA 45.0 45.0 58.0 58.1 1429 bp 0.5 59.2
rpl32-ndhF_p5 TTAGACTTGCCGGTCGAAAG TACATGAAGGGGGAATTGCG 50.0 50.0 57.9 57.9 1995 bp 0.5 59.0