Hotspot

trnL (UAG)-rpl32

SSC · 123383-124075 · 693 bp

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Pi 0.0893
MarkerSeek score 79.60
Primer available yes
Label trnL (UAG)-rpl32

Feature Metadata

label_name
trnL (UAG)-rpl32
feature_type
igs
region
SSC
length_bp
693
variable_sites
61
pi
0.0893
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL (UAG)-rpl32_p1 AGCGTGTCTACCGATTTCAC GCGGATTCGTTGGACTAGTT 50.0 50.0 58.0 58.0 1336 bp 0.5 59.9
trnL (UAG)-rpl32_p2 CAGCGTGTCTACCGATTTCA GCGGATTCGTTGGACTAGTT 50.0 50.0 58.0 58.0 1337 bp 0.5 59.9
trnL (UAG)-rpl32_p3 GGTGTTTTGAAGACGGCATG GCGGATTCGTTGGACTAGTT 50.0 50.0 57.9 58.0 1405 bp 0.5 59.5
trnL (UAG)-rpl32_p4 AGCGTGTCTACCGATTTCAC CTTTCGACCGGCAAGTCTAA 50.0 50.0 58.0 57.9 776 bp 0.5 59.3
trnL (UAG)-rpl32_p5 AGCGTGTCTACCGATTTCAC TCGACCGGCAAGTCTAAAAG 50.0 50.0 58.0 57.9 773 bp 0.5 59.3