Hotspot

trnI (CAU)-ycf2

IRb · 84991-85112 · 122 bp

Back to Pratia

Pi 0.0331
MarkerSeek score 72.30
Primer available yes
Label trnI (CAU)-ycf2

Feature Metadata

label_name
trnI (CAU)-ycf2
feature_type
igs
region
IRb
length_bp
122
variable_sites
4
pi
0.0331
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI (CAU)-ycf2_p1 TGAACCTACGAATTCGCCAA CAGAACCTTACGAAAGATTGCG 45.0 45.5 57.5 58.0 265-266 bp 1.0 61.8
trnI (CAU)-ycf2_p2 TTGAACCTACGAATTCGCCA CAGAACCTTACGAAAGATTGCG 45.0 45.5 57.5 58.0 266-267 bp 1.0 61.8
trnI (CAU)-ycf2_p3 TGAACCTACGAATTCGCCAA AGAACCTTACGAAAGATTGCGA 45.0 40.9 57.5 58.1 264-265 bp 1.0 61.2
trnI (CAU)-ycf2_p4 TTGAACCTACGAATTCGCCA AGAACCTTACGAAAGATTGCGA 45.0 40.9 57.5 58.1 265-266 bp 1.0 61.2
trnI (CAU)-ycf2_p5 GCCAATTATGAGTTGGGTGC CAGAACCTTACGAAAGATTGCG 50.0 45.5 57.4 58.0 250-251 bp 1.0 60.8