Hotspot

rpoA-rps11

LSC · 77212-77291 · 80 bp

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Pi 0.05
MarkerSeek score 76.30
Primer available yes
Label rpoA-rps11

Feature Metadata

label_name
rpoA-rps11
feature_type
igs
region
LSC
length_bp
80
variable_sites
4
pi
0.05
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA-rps11_p1 CACACTTCCACTGGAGTGTC GCCCCATAATGGCTGTAGAC 55.0 55.0 58.1 58.1 175 bp 1.0 80.0
rpoA-rps11_p2 CACACTTCCACTGGAGTGTC ACGGGATGTAACTCCTTTGC 55.0 50.0 58.1 57.9 193 bp 1.0 79.8
rpoA-rps11_p3 CACACTTCCACTGGAGTGTC GGATGTAACTCCTTTGCCCC 55.0 55.0 58.1 58.2 190 bp 1.0 78.8
rpoA-rps11_p4 CACACTTCCACTGGAGTGTC ACGCAGCATTACGAGCTATT 55.0 45.0 58.1 57.8 242 bp 1.0 78.5
rpoA-rps11_p5 CACACTTCCACTGGAGTGTC GACGCAGCATTACGAGCTAT 55.0 50.0 58.1 58.2 243 bp 1.0 78.5