Hotspot

ndhC-ORF224

LSC · 56238-56544 · 307 bp

Back to Pratia

Pi 0.0903
MarkerSeek score 74.50
Primer available yes
Label ndhC-ORF224

Feature Metadata

label_name
ndhC-ORF224
feature_type
igs
region
LSC
length_bp
307
variable_sites
27
pi
0.0903
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-ORF224_p1 CTAGAAAGTTTCTCCGGCCC CGGGCAAAGAAGAAATGCAG 55.0 50.0 58.0 58.0 515-670 bp 1.0 84.7
ndhC-ORF224_p2 CTAGAAAGTTTCTCCGGCCC GTCGGGCAAAGAAGAAATGC 55.0 50.0 58.0 58.0 517-672 bp 1.0 84.6
ndhC-ORF224_p3 GTGAGCCGATTAGTGAAGCA CGGGCAAAGAAGAAATGCAG 50.0 50.0 58.0 58.0 962-1117 bp 1.0 82.9
ndhC-ORF224_p4 GTGAGCCGATTAGTGAAGCA GTCGGGCAAAGAAGAAATGC 50.0 50.0 58.0 58.0 964-1119 bp 1.0 82.9
ndhC-ORF224_p5 GTGAGCCGATTAGTGAAGCA ACGTCTGAAAAACCCCTCAC 50.0 50.0 58.0 58.0 1331 bp 0.5 59.6