Hotspot

trnT (UGU)-trnL (UAA)

LSC · 52277-52955 · 679 bp

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Pi 0.0433
MarkerSeek score 77.30
Primer available yes
Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name
trnT (UGU)-trnL (UAA)
feature_type
igs
region
LSC
length_bp
679
variable_sites
29
pi
0.0433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT (UGU)-trnL (UAA)_p1 GCGATGCTCTACCTTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 725-729 bp 1.0 83.2
trnT (UGU)-trnL (UAA)_p2 GCGATGCTCTACCTTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 733-737 bp 1.0 82.6
trnT (UGU)-trnL (UAA)_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 757-761 bp 1.0 82.1
trnT (UGU)-trnL (UAA)_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 765-769 bp 1.0 81.6
trnT (UGU)-trnL (UAA)_p5 GCGATGCTCTACCTTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 739-743 bp 1.0 81.2