Hotspot

trnC (GCA)-petN

LSC · 34522-34788 · 267 bp

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Pi 0.0498
MarkerSeek score 73.10
Primer available yes
Label trnC (GCA)-petN

Feature Metadata

label_name
trnC (GCA)-petN
feature_type
igs
region
LSC
length_bp
267
variable_sites
13
pi
0.0498
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC (GCA)-petN_p1 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 341-342 bp 1.0 80.4
trnC (GCA)-petN_p2 GGGGGACTGCAAATCCTTTT CCCAAGCGAGACTTACTAGC 50.0 55.0 58.4 57.8 339-340 bp 1.0 79.9
trnC (GCA)-petN_p3 GGGGGACTGCAAATCCTTTT GCCCAAGCGAGACTTACTAG 50.0 55.0 58.4 57.8 340-341 bp 1.0 79.9
trnC (GCA)-petN_p4 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.5 58.2 316-317 bp 1.0 79.8
trnC (GCA)-petN_p5 AGTTCAAATCTGGGTGTCGC CCCAAGCGAGACTTACTAGC 50.0 55.0 58.5 57.8 314-315 bp 1.0 79.3