Hotspot

ORF238-trnC (GCA)

LSC · 33597-34450 · 854 bp

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Pi 0.145
MarkerSeek score 72.60
Primer available yes
Label ORF238-trnC (GCA)

Feature Metadata

label_name
ORF238-trnC (GCA)
feature_type
igs
region
LSC
length_bp
854
variable_sites
75
pi
0.145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.61
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF238-trnC (GCA)_p1 GGTCTGAAAACATTTCCGCG GAAGCCTGTTGAGGAACCAA 50.0 50.0 58.0 58.0 1612 bp 0.5 59.6
ORF238-trnC (GCA)_p2 GGTCTGAAAACATTTCCGCG GGTGCAGAGTCAAAAAGGGA 50.0 50.0 58.0 58.0 1640 bp 0.5 59.6
ORF238-trnC (GCA)_p3 GGTTTGTCAGGTTCTGGTGT GAAGCCTGTTGAGGAACCAA 50.0 50.0 57.9 58.0 1298 bp 0.5 59.4
ORF238-trnC (GCA)_p4 GGTTTGTCAGGTTCTGGTGT GGTGCAGAGTCAAAAAGGGA 50.0 50.0 57.9 58.0 1326 bp 0.5 59.4
ORF238-trnC (GCA)_p5 TTCTGGTGTCCCTCCTGTAG GAAGCCTGTTGAGGAACCAA 55.0 50.0 58.1 58.0 1287 bp 0.5 59.4