Hotspot

trnS (GCU)-trnG (UCC)

LSC · 14018-14756 · 739 bp

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Pi 0.043
MarkerSeek score 74.30
Primer available yes
Label trnS (GCU)-trnG (UCC)

Feature Metadata

label_name
trnS (GCU)-trnG (UCC)
feature_type
igs
region
LSC
length_bp
739
variable_sites
31
pi
0.043
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS (GCU)-trnG (UCC)_p1 ACGGAAAGAGAGGGATTCGA AGGAGGTTCATAGAACGAATCAC 50.0 43.5 58.2 58.0 860-876 bp 1.0 59.2
trnS (GCU)-trnG (UCC)_p2 ACGAAAACGGAAAGAGAGGG AGGAGGTTCATAGAACGAATCAC 50.0 43.5 57.8 58.0 866-882 bp 1.0 59.1
trnS (GCU)-trnG (UCC)_p3 ACGGAAAGAGAGGGATTCGA GGAGGTTCATAGAACGAATCACA 50.0 43.5 58.2 58.2 859-875 bp 1.0 57.4
trnS (GCU)-trnG (UCC)_p4 ACGAAAACGGAAAGAGAGGG GGAGGTTCATAGAACGAATCACA 50.0 43.5 57.8 58.2 865-881 bp 1.0 57.2
trnS (GCU)-trnG (UCC)_p5 AGTCATCGACGAAAACGGAA AGGAGGTTCATAGAACGAATCAC 45.0 43.5 57.6 58.0 874-890 bp 1.0 56.9