Hotspot

psbA-rbcL

LSC · 1497-2538 · 1042 bp

Back to Pratia

Pi 0.0358
MarkerSeek score 80.10
Primer available yes
Label psbA-rbcL

Feature Metadata

label_name
psbA-rbcL
feature_type
igs
region
LSC
length_bp
1042
variable_sites
37
pi
0.0358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-rbcL_p1 TACAGAAGCGACCCCATAGG ATTTGAGTTTGCCGCAATGG 55.0 45.0 58.6 57.9 1128-1129 bp 1.0 77.9
psbA-rbcL_p2 TACAGAAGCGACCCCATAGG TGAGTTTGCCGCAATGGATA 55.0 45.0 58.6 57.9 1125-1126 bp 1.0 77.6
psbA-rbcL_p3 TACAGAAGCGACCCCATAGG GCTGCTTGTGAGGTATGGAA 55.0 50.0 58.6 57.9 1157-1158 bp 1.0 77.5
psbA-rbcL_p4 TACAGAAGCGACCCCATAGG AGTTTGCCGCAATGGATACT 55.0 45.0 58.6 57.9 1123-1124 bp 1.0 77.5
psbA-rbcL_p5 ATCCAGTTACAGAAGCGACC ATTTGAGTTTGCCGCAATGG 50.0 45.0 57.3 57.9 1135-1136 bp 1.0 77.2