Hotspot

rpl2

LSC · 84707-86181 · 1475 bp

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Pi 0.00746

MarkerSeek score 63.00

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1475
variable_sites: 11
pi: 0.00746
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	AACGTGTCACAGCTTACTCC	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	1611-1613 bp	1.0	77.2
rpl2_p2	AACGTGTCACAGCTTACTCC	ATCATTACGCTTCAACCGGG	50.0	50.0	57.8	58.3	1618-1620 bp	1.0	76.6
rpl2_p3	AACGTGTCACAGCTTACTCC	ACGCTTCAACCGGGTTATTC	50.0	50.0	57.8	58.6	1612-1614 bp	1.0	74.9
rpl2_p4	AACGTGTCACAGCTTACTCC	TACGCTTCAACCGGGTTATT	50.0	45.0	57.8	57.2	1613-1615 bp	1.0	73.2
rpl2_p5	AACGTGTCACAGCTTACTCC	TTACGCTTCAACCGGGTTAT	50.0	45.0	57.8	57.2	1614-1616 bp	1.0	73.2