Hotspot

clpP

LSC · 70339-72416 · 2078 bp

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Pi 0.0107

MarkerSeek score 58.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2078
variable_sites: 22
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGGAAATCTATAATTTGCGCGA	ACGTTTCCACATCAAAGTGA	36.4	40.0	56.3	55.5	2161-2169 bp	1.0	41.4
clpP_p2	TGGTTTAGATCGATCCTAACCTG	ACGTTTCCACATCAAAGTGA	43.5	40.0	57.5	55.5	2240-2248 bp	1.0	41.3
clpP_p3	AGATCGATCCTAACCTGATGA	ACGTTTCCACATCAAAGTGA	42.9	40.0	55.4	55.5	2234-2242 bp	1.0	41.3
clpP_p4	TGGTTTAGATCGATCCTAACCTG	ACGTTTCCACATCAAAGTGAA	43.5	38.1	57.5	56.3	2240-2248 bp	1.0	41.3
clpP_p5	GGTTTAGATCGATCCTAACCTGA	ACGTTTCCACATCAAAGTGA	43.5	40.0	57.2	55.5	2239-2247 bp	1.0	41.3