Hotspot

ndhI-ndhA

SSC · 120641-120733 · 93 bp

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Pi 0.0215

MarkerSeek score 64.90

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 93
variable_sites: 2
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGCAGCTCGTACTGTTTGTT	CCAAGGCTAAGAATGGACCA	45.0	50.0	58.1	57.2	258 bp	1.0	73.7
ndhI-ndhA_p2	TTGCAGCTCGTACTGTTTGT	CCAAGGCTAAGAATGGACCA	45.0	50.0	58.1	57.2	259 bp	1.0	73.7
ndhI-ndhA_p3	TGCAGCTCGTACTGTTTGTT	ACCAAGGCTAAGAATGGACC	45.0	50.0	58.1	57.2	259 bp	1.0	73.7
ndhI-ndhA_p4	TTGCAGCTCGTACTGTTTGT	ACCAAGGCTAAGAATGGACC	45.0	50.0	58.1	57.2	260 bp	1.0	73.7
ndhI-ndhA_p5	TTGACCGATGTACCTTGCAG	CCAAGGCTAAGAATGGACCA	50.0	50.0	57.9	57.2	273 bp	1.0	73.4