Hotspot

rps8-rpl14

LSC · 80957-81157 · 201 bp

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Pi 0.0398
MarkerSeek score 65.00
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
201
variable_sites
8
pi
0.0398
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TGCTATTCGAACCACCCTTT CCCGGGAATTGAGACAGTTA 45.0 50.0 57.1 56.9 344 bp 1.0 66.3
rps8-rpl14_p2 ATGCTATTCGAACCACCCTT CCCGGGAATTGAGACAGTTA 45.0 50.0 56.9 56.9 345 bp 1.0 64.5
rps8-rpl14_p3 TGCTATTCGAACCACCCTTT CCCGGGAATTGAGACAGTTAA 45.0 47.6 57.1 57.7 344 bp 1.0 64.1
rps8-rpl14_p4 TGCTATTCGAACCACCCTTTT CCCGGGAATTGAGACAGTTA 42.9 50.0 57.9 56.9 344 bp 1.0 64.1
rps8-rpl14_p5 ATGCTATTCGAACCACCCTTT CCCGGGAATTGAGACAGTTA 42.9 50.0 57.6 56.9 345 bp 1.0 62.4