Hotspot

accD-psaI

LSC · 59673-60171 · 499 bp

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Pi 0.0164

MarkerSeek score 68.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 8
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.8	57.9	610-622 bp	1.0	75.7
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	50.0	45.0	58.8	58.2	600-612 bp	1.0	75.3
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	52.6	45.0	58.1	57.9	610-622 bp	1.0	73.2
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	42.9	45.0	58.1	57.9	608-620 bp	1.0	73.2
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	AAAGAAGCCATTGCCATTGC	50.0	45.0	56.9	57.9	621-633 bp	1.0	73.0