Hotspot

ndhC-trnV-UAC

LSC · 51304-52418 · 1115 bp

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Pi 0.00987

MarkerSeek score 64.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1115
variable_sites: 11
pi: 0.00987
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1267-1295 bp	1.0	78.8
ndhC-trnV-UAC_p2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1254-1282 bp	1.0	75.0
ndhC-trnV-UAC_p3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1252-1280 bp	1.0	74.2
ndhC-trnV-UAC_p4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.3	58.1	1262-1290 bp	1.0	74.1
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.8	58.1	1249-1277 bp	1.0	73.2