Hotspot

trnT-GGU-psbD

LSC · 32017-33450 · 1434 bp

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Pi 0.0128

MarkerSeek score 59.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1434
variable_sites: 18
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	50.0	50.0	58.1	58.1	1593-1617 bp	1.0	81.4
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1589-1613 bp	1.0	81.2
trnT-GGU-psbD_p3	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	50.0	55.0	58.1	57.8	1600-1624 bp	1.0	80.6
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	50.0	55.0	57.9	57.8	1596-1620 bp	1.0	80.4
trnT-GGU-psbD_p5	AAGGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	50.0	50.0	58.1	58.2	1601-1625 bp	1.0	80.2