Hotspot

trnE-UUC-trnT-GGU

LSC · 31254-31944 · 691 bp

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Pi 0.0147
MarkerSeek score 63.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
691
variable_sites
10
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 795-815 bp 1.0 80.5
trnE-UUC-trnT-GGU_p2 GTGTAGTACCCTACCCCCAG GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 830-850 bp 1.0 80.0
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 760-780 bp 1.0 79.8
trnE-UUC-trnT-GGU_p4 GTGTAGTACCCTACCCCCAG TACCGCTGAGTTAAAAGGGC 60.0 50.0 58.2 57.9 795-815 bp 1.0 79.3
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 792-812 bp 1.0 79.3