Hotspot

rpoB-trnC-GCA

LSC · 26814-28049 · 1236 bp

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Pi 0.0149

MarkerSeek score 64.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1236
variable_sites: 18
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGCCCTGATCCATGAACCTA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1335-1349 bp	1.0	80.7
rpoB-trnC-GCA_p2	AGCCCTGATCCATGAACCTA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1375-1389 bp	1.0	80.5
rpoB-trnC-GCA_p3	GCCCTGATCCATGAACCTAC	CTTACCACTCGGCCATATCG	55.0	55.0	57.7	57.9	1334-1348 bp	1.0	80.2
rpoB-trnC-GCA_p4	GCCCTGATCCATGAACCTAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.7	58.1	1374-1388 bp	1.0	80.0
rpoB-trnC-GCA_p5	AAAGTTCTTCCGTCAAGCCC	CTTACCACTCGGCCATATCG	50.0	55.0	58.4	57.9	1350-1364 bp	1.0	79.1