Hotspot

psbK-psbI

LSC · 7991-8314 · 324 bp

Back to Prangos

Pi 0.0252

MarkerSeek score 61.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 8
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	439-444 bp	1.0	81.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	440-445 bp	1.0	81.5
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	50.0	50.0	57.9	58.3	441-446 bp	1.0	79.7
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.3	58.1	436-441 bp	1.0	79.5
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.3	58.1	437-442 bp	1.0	79.5