Hotspot

rps16-trnQ-UUG

LSC · 6197-7381 · 1185 bp

Back to Prangos

Pi 0.0152
MarkerSeek score 57.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1185
variable_sites
15
pi
0.0152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1114-1304 bp 1.0 83.1
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1115-1305 bp 1.0 83.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 1125-1315 bp 1.0 82.3
rps16-trnQ-UUG_p4 TGCTTTCTACCACAGCGTTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1078-1268 bp 1.0 80.5
rps16-trnQ-UUG_p5 TTGCTTTCTACCACAGCGTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1079-1269 bp 1.0 80.5