Hotspot

ycf1

SSC · 123765-129524 · 5760 bp

Back to Pouzolzia

Pi 0.0628

MarkerSeek score 73.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5760
variable_sites: 519
pi: 0.0628
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAACGAGCCTGCGATTATGT	TTTTCGCCGTCAATCGAAGA	45.0	45.0	58.0	58.2	618 bp	0.333333	51.4
ycf1_p2	TTGAGCGAATCGGCGAATAA	TTTTCGCCGTCAATCGAAGA	45.0	45.0	58.1	58.2	454 bp	0.333333	51.1
ycf1_p3	TGAGCGAATCGGCGAATAAT	TTTTCGCCGTCAATCGAAGA	45.0	45.0	57.9	58.2	453 bp	0.333333	50.4
ycf1_p4	AGCAAGTGCAAACCTATCCC	TTTTCGCCGTCAATCGAAGA	50.0	45.0	58.2	58.2	924 bp	0.333333	50.3
ycf1_p5	TTCGTCGTTTTCGTTACCGT	TTTTCGCCGTCAATCGAAGA	45.0	45.0	58.2	58.2	420 bp	0.333333	50.3