Hotspot

ndhF-rpl32

SSC · 111928-113077 · 1150 bp

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Pi 0.0601
MarkerSeek score 85.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1150
variable_sites
97
pi
0.0601
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGGGAATAAAAGGTAAAATCCA AAAAGGCTTTCAATGCTGCC 34.8 45.0 55.5 57.8 1263-1294 bp 1.0 50.1
ndhF-rpl32_p2 GGGGAATAAAAGGTAAAATCCATGA AAAAGGCTTTCAATGCTGCC 36.0 45.0 57.3 57.8 1262-1293 bp 1.0 50.1
ndhF-rpl32_p3 AGGGGAATAAAAGGTAAAATCCATG AAAAGGCTTTCAATGCTGCC 36.0 45.0 57.3 57.8 1263-1294 bp 1.0 50.1
ndhF-rpl32_p4 AGGGGAATAAAAGGTAAAATCCAT AAAAGGCTTTCAATGCTGCC 33.3 45.0 56.0 57.8 1263-1294 bp 1.0 50.1
ndhF-rpl32_p5 GGGGAATAAAAGGTAAAATCCATG AAAAGGCTTTCAATGCTGCC 37.5 45.0 56.0 57.8 1262-1293 bp 1.0 50.1