Hotspot

psbE-petL

LSC · 64242-65349 · 1108 bp

Back to Pouzolzia

Pi 0.0531

MarkerSeek score 80.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1108
variable_sites: 83
pi: 0.0531
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1760-1906 bp	1.0	89.2
psbE-petL_p2	ACGGTAGGTACAGCTAGTCC	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.0	58.0	1980-2126 bp	1.0	88.4
psbE-petL_p3	GGTAGGTACAGCTAGTCCGT	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.0	58.0	1978-2124 bp	1.0	88.4
psbE-petL_p4	TCGTTCGGGTTTGATTGTGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	2071-2217 bp	1.0	88.3
psbE-petL_p5	TTCGGGTTTGATTGTGTCGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	2068-2214 bp	1.0	88.3