Hotspot

trnG-GCC-trnfM-CAU

LSC · 36008-36196 · 189 bp

Back to Pouzolzia

Pi 0.0618
MarkerSeek score 81.10
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
189
variable_sites
16
pi
0.0618
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 248-255 bp 1.0 88.3
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 249-256 bp 1.0 88.3
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 246-253 bp 1.0 87.2
trnG-GCC-trnfM-CAU_p4 AGAAGATGCGGGTTCGATTC GCTTGGTAGCTTACAAGGCT 50.0 50.0 58.1 57.9 274-281 bp 1.0 87.0
trnG-GCC-trnfM-CAU_p5 GAGAAGATGCGGGTTCGATT GCTTGGTAGCTTACAAGGCT 50.0 50.0 58.1 57.9 275-282 bp 1.0 86.9