Hotspot

rpoB-trnC-GCA

LSC · 26297-27299 · 1003 bp

Back to Pouzolzia

Pi 0.065

MarkerSeek score 82.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1003
variable_sites: 92
pi: 0.065
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1577-1763 bp	1.0	88.2
rpoB-trnC-GCA_p2	ACTCCACAACGGCATTTTCT	CCGGATTTGAACTGGGGAAA	45.0	50.0	58.0	58.1	1747-1933 bp	1.0	87.9
rpoB-trnC-GCA_p3	TACCGGATCTCCACCTACAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1785-1971 bp	1.0	87.5
rpoB-trnC-GCA_p4	ACTCCACAACGGCATTTTCT	AATCTACGCTTGGCTTGCTT	45.0	45.0	58.0	58.2	1844 bp	0.333333	51.8
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	AATCTACGCTTGGCTTGCTT	50.0	45.0	58.0	58.2	1674 bp	0.333333	51.7