Hotspot

ycf1

SSC · 128286-133928 · 5643 bp

Back to Pouteria

Pi 0.00744

MarkerSeek score 45.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5643
variable_sites: 63
pi: 0.00744
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCATCCAGATAAGAAGCTTCA	GCAGAATACCGTCACCCATT	45.5	50.0	57.6	58.0	275-302 bp	1.0	62.2
ycf1_p2	TCCCATCCAGATAAGAAGCTTC	GCAGAATACCGTCACCCATT	45.5	50.0	57.3	58.0	276-303 bp	1.0	60.0
ycf1_p3	TCCCATCCAGATAAGAAGCT	GCAGAATACCGTCACCCATT	45.0	50.0	55.3	58.0	276-303 bp	1.0	59.9
ycf1_p4	TCCCATCCAGATAAGAAGCTT	GCAGAATACCGTCACCCATT	42.9	50.0	56.1	58.0	276-303 bp	1.0	58.5
ycf1_p5	TTCCCATCCAGATAAGAAGCT	GCAGAATACCGTCACCCATT	42.9	50.0	56.1	58.0	277-304 bp	1.0	58.5