Hotspot

rps15-ycf1

SSC · 127910-128285 · 376 bp

Back to Pouteria

Pi 0.0177
MarkerSeek score 54.40
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
376
variable_sites
10
pi
0.0177
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CCTCGGTTTTCTTCTTTTTCT AGGATACATATATATCACGATTGA 38.1 29.2 54.3 52.5 459-464 bp 1.0 42.6
rps15-ycf1_p2 CCTCGGTTTTCTTCTTTTTCTT AGGATACATATATATCACGATTGA 36.4 29.2 55.1 52.5 459-464 bp 1.0 42.6
rps15-ycf1_p3 CCTCGGTTTTCTTCTTTTTCT AAGGATACATATATATCACGATTGA 38.1 28.0 54.3 53.3 460-465 bp 1.0 42.6
rps15-ycf1_p4 CCTCGGTTTTCTTCTTTTTCTT AAGGATACATATATATCACGATTGA 36.4 28.0 55.1 53.3 460-465 bp 1.0 42.6
rps15-ycf1_p5 CCTCGGTTTTCTTCTTTTTCTTT AGGATACATATATATCACGATTGA 34.8 29.2 55.9 52.5 459-464 bp 1.0 42.6