Hotspot

psbB-psbT

LSC · 77720-77909 · 190 bp

Back to Pouteria

Pi 0.0106

MarkerSeek score 62.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 3
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGTGCTAGAACCCTGTTCAG	GAACTTTAGGCGGTTCTCGA	55.0	50.0	57.9	57.6	393-398 bp	1.0	78.1
psbB-psbT_p2	GGTGCTAGAACCCTGTTCAG	TGGAACTTTAGGCGGTTCTC	55.0	50.0	57.9	57.5	395-400 bp	1.0	77.3
psbB-psbT_p3	TGGATGCCCAAGTGGAATTT	GAACTTTAGGCGGTTCTCGA	45.0	50.0	57.7	57.6	344-349 bp	1.0	76.6
psbB-psbT_p4	TTGGATGCCCAAGTGGAATT	GAACTTTAGGCGGTTCTCGA	45.0	50.0	57.7	57.6	345-350 bp	1.0	76.6
psbB-psbT_p5	GGTGCTAGAACCCTGTTCAG	GGAACTTTAGGCGGTTCTCG	55.0	55.0	57.9	58.6	394-399 bp	1.0	75.9