Hotspot

psbE-petL

LSC · 68183-69467 · 1285 bp

Back to Pouteria

Pi 0.00808

MarkerSeek score 65.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 15
pi: 0.00808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	981-1477 bp	1.0	77.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	904-1400 bp	1.0	76.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	990-1486 bp	1.0	76.0
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	995-1491 bp	1.0	75.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	989-1485 bp	1.0	75.9