Hotspot

petA-psbJ

LSC · 66308-67402 · 1095 bp

Back to Pouteria

Pi 0.0306

MarkerSeek score 82.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 50
pi: 0.0306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1183-1205 bp	1.0	81.1
petA-psbJ_p2	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1184-1206 bp	1.0	78.8
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	TGGTTCATATTCCGGATTGGG	45.0	47.6	57.8	57.8	1191-1213 bp	1.0	74.2
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	GGTTCATATTCCGGATTGGGT	45.0	47.6	57.8	57.8	1190-1212 bp	1.0	74.2
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	1133-1155 bp	1.0	73.9