Hotspot

rpoB-trnC-GCA

LSC · 27639-28887 · 1249 bp

Back to Pouteria

Pi 0.00267

MarkerSeek score 64.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1249
variable_sites: 5
pi: 0.00267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCCGTTAAGCCCTGA	TTATTGATCAGGCGACACCC	50.0	50.0	57.5	57.7	1401-1432 bp	1.0	76.1
rpoB-trnC-GCA_p2	AGTTCTTCCGTTAAGCCCTG	TTATTGATCAGGCGACACCC	50.0	50.0	57.5	57.7	1402-1433 bp	1.0	76.1
rpoB-trnC-GCA_p3	GTTCTTCCGTTAAGCCCTGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.5	58.4	1365-1396 bp	1.0	76.0
rpoB-trnC-GCA_p4	AGTTCTTCCGTTAAGCCCTG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.5	58.4	1366-1397 bp	1.0	75.9
rpoB-trnC-GCA_p5	GTTCTTCCGTTAAGCCCTGA	GCGACACCCAGATTTGAACT	50.0	50.0	57.5	58.5	1390-1421 bp	1.0	74.9