Hotspot

atpH-atpI

LSC · 14239-15382 · 1144 bp

Back to Pouteria

Pi 0.00644

MarkerSeek score 64.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 11
pi: 0.00644
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1222-1232 bp	1.0	77.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	AGGTGAATCTATGGAGGGCC	45.0	55.0	58.0	58.6	1194-1204 bp	1.0	76.7
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1278-1288 bp	1.0	76.4
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	AGGTGAATCTATGGAGGGCC	50.0	55.0	57.9	58.6	1250-1260 bp	1.0	76.1
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1223-1233 bp	1.0	75.9