Hotspot

ycf3

LSC · 45722-47712 · 1991 bp

Back to Pourthiaea

Pi 0.00251

MarkerSeek score 35.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1991
variable_sites: 5
pi: 0.00251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACACATACAAAGAGACCCGC	AAATTCATGAACCGTCCCCC	50.0	50.0	57.9	58.2	80-2907 bp	1.0	79.9
ycf3_p2	CATACAAAGAGACCCGCCAA	AAATTCATGAACCGTCCCCC	50.0	50.0	57.9	58.2	80-2904 bp	1.0	79.8
ycf3_p3	TCTGGGCTTTAATTGACGCA	ATGTTTGCGAAGATGGGGTT	45.0	45.0	57.8	58.1	80-2906 bp	1.0	79.8
ycf3_p4	GATCTGGAAGTTGATCCGGG	ATGTTTGCGAAGATGGGGTT	55.0	45.0	57.8	58.1	80-2998 bp	1.0	79.3
ycf3_p5	TCTGGGCTTTAATTGACGCA	GGGTTTCCGCCCTTTCTAAT	45.0	50.0	57.8	57.9	80-2891 bp	1.0	79.3