Hotspot

rpl32-trnL-UAG

SSC · 118197-119636 · 1440 bp

Back to Pourthiaea

Pi 0.00444
MarkerSeek score 49.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1440
variable_sites
21
pi
0.00444
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 80-1590 bp 1.0 81.1
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 80-1591 bp 1.0 81.1
rpl32-trnL-UAG_p3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 80-1592 bp 1.0 80.8
rpl32-trnL-UAG_p4 TAGGGCAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 80-1593 bp 1.0 80.8
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 80-1600 bp 1.0 80.2