Hotspot

ndhC-trnV-UAC

LSC · 54505-55080 · 576 bp

Back to Pourthiaea

Pi 0.0146
MarkerSeek score 63.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
576
variable_sites
24
pi
0.0146
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.9 58.2 80-771 bp 1.0 84.4
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 80-707 bp 1.0 84.4
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AATGCACGTTGGGTCTTTGA 50.0 45.0 57.9 58.3 80-774 bp 1.0 83.2
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT ATGCACGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 80-773 bp 1.0 83.2
ndhC-trnV-UAC_p5 CTGGTCCTTTGCTAATCGGG GCACGTTGGGTCTTTGAAAC 55.0 50.0 58.3 58.2 80-788 bp 1.0 82.8