Hotspot

psaA-ycf3

LSC · 44987-45721 · 735 bp

Back to Pourthiaea

Pi 0.000707

MarkerSeek score 51.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 2
pi: 0.000707
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCAATAGCGCT	50.0	45.0	57.9	58.5	80-896 bp	1.0	78.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CAATAGCGCTTACTCCTGGT	50.0	50.0	57.9	57.4	80-886 bp	1.0	77.5
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	80-927 bp	1.0	77.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GCAATAGCGCTTACTCCTGG	50.0	55.0	57.9	58.8	80-887 bp	1.0	76.1
psaA-ycf3_p5	TTGAGAAATGACCCGGTCTG	TGGAAACAAGCAATAGCGCT	50.0	45.0	57.5	58.5	80-916 bp	1.0	75.6