Hotspot

trnT-GGU-psbD

LSC · 34318-35919 · 1602 bp

Back to Pourthiaea

Pi 0.0116

MarkerSeek score 64.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1602
variable_sites: 58
pi: 0.0116
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	80-1859 bp	1.0	86.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	80-1862 bp	1.0	86.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	80-1861 bp	1.0	86.2
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	80-1849 bp	1.0	86.2
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	80-1864 bp	1.0	86.0