Hotspot

trnR-UCU-atpA

LSC · 10153-11058 · 906 bp

Back to Pourthiaea

Pi 0.0182

MarkerSeek score 58.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 42
pi: 0.0182
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	495-1074 bp	1.0	72.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	435-1014 bp	1.0	71.3
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	47.6	50.0	56.9	58.4	470-1049 bp	1.0	68.3
trnR-UCU-atpA_p4	GGAATGAAAAGCGTCCATTGT	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	496-1055 bp	1.0	66.8
trnR-UCU-atpA_p5	GGAATGAAAAGCGTCCATTGT	ACCAAGACATTTACCGACGA	42.9	45.0	57.4	56.9	495-1078 bp	1.0	66.0