Hotspot

trnH-GUG-psbA

LSC · 156-416 · 261 bp

Back to Pourthiaea

Pi 0.0111
MarkerSeek score 46.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
261
variable_sites
11
pi
0.0111
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 378-391 bp 1.0 81.8
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 392-405 bp 1.0 81.7
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 389-402 bp 0.875 76.9
trnH-GUG-psbA_p4 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 439-452 bp 0.875 76.8
trnH-GUG-psbA_p5 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 392-405 bp 0.875 76.8