Hotspot

petA-psbJ

LSC · 68694-69793 · 1100 bp

Back to Pothos

Pi 0.0111
MarkerSeek score 66.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1100
variable_sites
12
pi
0.0111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGCGTTCAAGGTCTTTTGTT CTGTGATCGGTTCAATCGGT 45.0 50.0 57.5 58.0 1272-1276 bp 1.0 77.9
petA-psbJ_p2 ACGCGTTCAAGGTCTTTTGT CTGTGATCGGTTCAATCGGT 45.0 50.0 58.6 58.0 1273-1277 bp 1.0 76.7
petA-psbJ_p3 CGCGTTCAAGGTCTTTTGTT TTCCTCTTTGGCTGGTAGGT 45.0 50.0 57.5 58.3 1308-1312 bp 1.0 75.9
petA-psbJ_p4 CGCGTTCAAGGTCTTTTGTT TCCTGTGATCGGTTCAATCG 45.0 50.0 57.5 57.7 1274-1278 bp 1.0 75.7
petA-psbJ_p5 CGTTACGCGTTCAAGGTCTT CTGTGATCGGTTCAATCGGT 50.0 50.0 58.9 58.0 1277-1281 bp 1.0 74.7