Hotspot

trnM-CAU-atpE

LSC · 57170-57328 · 159 bp

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Pi 0.0629
MarkerSeek score 82.80
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
159
variable_sites
10
pi
0.0629
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGCAGGAGTCATTGGTTCAA CTCTCAGACGAGCTAGGACA 50.0 55.0 58.1 58.0 253 bp 1.0 82.2
trnM-CAU-atpE_p2 GGCAGGAGTCATTGGTTCAA GCGAATCTAGCTCTCAGACG 50.0 55.0 58.1 58.0 263 bp 1.0 82.1
trnM-CAU-atpE_p3 GGCAGGAGTCATTGGTTCAA TGAGGCGAATCTAGCTCTCA 50.0 50.0 58.1 57.9 267 bp 1.0 81.8
trnM-CAU-atpE_p4 GGCAGGAGTCATTGGTTCAA AAGACAAGCAATTGAGGCGA 50.0 45.0 58.1 58.1 279 bp 1.0 81.3
trnM-CAU-atpE_p5 GGCAGGAGTCATTGGTTCAA AGACAAGCAATTGAGGCGAA 50.0 45.0 58.1 58.1 278 bp 1.0 81.3