Hotspot

trnF-GAA-ndhJ

LSC · 52272-53210 · 939 bp

Back to Pothos

Pi 0.0145
MarkerSeek score 61.90
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
939
variable_sites
11
pi
0.0145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 902-1049 bp 1.0 83.5
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATCATCCACGCCTTAAACGT 50.0 45.0 58.1 57.6 933-1080 bp 1.0 81.3
trnF-GAA-ndhJ_p3 TCGGGATAGCTCAGTTGGTA TGAAAGTTGGATAGGCTGGC 50.0 50.0 57.6 57.9 940-1087 bp 1.0 80.3
trnF-GAA-ndhJ_p4 ATGCATGGAAAATGGTCGGT TGAAAGTTGGATAGGCTGGC 45.0 50.0 58.1 57.9 1106 bp 0.5 57.8
trnF-GAA-ndhJ_p5 ATGCATGGAAAATGGTCGGT ATCATCCACGCCTTAAACGT 45.0 45.0 58.1 57.6 1137 bp 0.5 55.6