Hotspot

petN-psbM

LSC · 30562-31738 · 1177 bp

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Pi 0.0146
MarkerSeek score 68.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1177
variable_sites
17
pi
0.0146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CTCGTAGTATGGGGGAGGAG TGCTACTGCACTGTTCATTCT 60.0 42.9 58.1 57.6 1285-1286 bp 1.0 69.8
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1338-1339 bp 1.0 69.2
petN-psbM_p3 GTAGTATGGGGGAGGAGTGG TGCTACTGCACTGTTCATTCT 60.0 42.9 58.3 57.6 1282-1283 bp 1.0 68.2
petN-psbM_p4 TCACTCGTAGTATGGGGGAG TGCTACTGCACTGTTCATTCT 55.0 42.9 57.6 57.6 1288-1289 bp 1.0 67.6
petN-psbM_p5 TAGTATGGGGGAGGAGTGGA TGCTACTGCACTGTTCATTCT 55.0 42.9 58.4 57.6 1281-1282 bp 1.0 67.5