Hotspot

atpF-atpH

LSC · 14451-14949 · 499 bp

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Pi 0.0294
MarkerSeek score 66.20
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
499
variable_sites
14
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GGCCAGGGAAACGAAAGAAT AATTTACGGACTGGTCGTGG 50.0 50.0 58.5 57.9 590-599 bp 1.0 80.5
atpF-atpH_p2 GCCAGGGAAACGAAAGAATC AATTTACGGACTGGTCGTGG 50.0 50.0 57.1 57.9 589-598 bp 1.0 76.8
atpF-atpH_p3 GGCCAGGGAAACGAAAGAA AATTTACGGACTGGTCGTGG 52.6 50.0 58.0 57.9 590-599 bp 1.0 75.9
atpF-atpH_p4 GGCCAGGGAAACGAAAGAAT ACAATTTACGGACTGGTCGT 50.0 45.0 58.5 57.2 592-601 bp 1.0 74.6
atpF-atpH_p5 GGCCAGGGAAACGAAAGAAT GGTCGTGGCATTAGCACTTT 50.0 50.0 58.5 58.8 578-587 bp 1.0 74.6