Hotspot

trnS-GCU-trnR-UCU

LSC · 9220-11256 · 2037 bp

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Pi 0.0208
MarkerSeek score 62.90
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
2037
variable_sites
42
pi
0.0208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 AGTGACCAGAGAAACGGAGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 2175-2236 bp 1.0 65.6
trnS-GCU-trnR-UCU_p2 ATTAGCAATCCGCCGCTTTA AGAAGACCTCTGTCCTATCCA 45.0 47.6 58.0 56.9 2113-2174 bp 1.0 65.4
trnS-GCU-trnR-UCU_p3 AGTGACCAGAGAAACGGAGA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 2181-2242 bp 1.0 65.0
trnS-GCU-trnR-UCU_p4 ATTAGCAATCCGCCGCTTTA AGGTTTAGAAGACCTCTGTCCT 45.0 45.5 58.0 57.9 2119-2180 bp 1.0 64.7
trnS-GCU-trnR-UCU_p5 GAGAAACGGAGAGAGAGGGA AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.9 56.9 2167-2228 bp 1.0 64.6