Hotspot

trnK-UUU-rps16

LSC · 4356-5232 · 877 bp

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Pi 0.0233
MarkerSeek score 63.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
877
variable_sites
20
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAGGAGCTCAACCCACAAG 50.0 50.0 58.2 58.0 975-1058 bp 1.0 81.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAGGAGCTCAACCCACAAG 50.0 50.0 58.2 58.0 974-1057 bp 1.0 81.5
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 970-1053 bp 1.0 81.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 969-1052 bp 1.0 81.4
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAGGAGCTCAACCCACAAG 50.0 50.0 58.3 58.0 984-1067 bp 1.0 80.5