Hotspot

psbA-trnK-UUU

LSC · 1490-1720 · 231 bp

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Pi 0.0223
MarkerSeek score 68.20
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
231
variable_sites
5
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TAGGCTTGTACTTTCGCGTC AATTGCAAGCACGATTTGGG 50.0 45.0 58.0 57.9 358-365 bp 1.0 81.9
psbA-trnK-UUU_p2 TAGGCTTGTACTTTCGCGTC CATAGGGAAAGTCGTGTGCA 50.0 50.0 58.0 57.9 383-390 bp 1.0 81.6
psbA-trnK-UUU_p3 ATGTAAAGGCGGTTTTCGGT AATTGCAAGCACGATTTGGG 45.0 45.0 58.1 57.9 408-415 bp 1.0 80.9
psbA-trnK-UUU_p4 ATGTAAAGGCGGTTTTCGGT CATAGGGAAAGTCGTGTGCA 45.0 50.0 58.1 57.9 433-440 bp 1.0 80.7
psbA-trnK-UUU_p5 CCATCCAATGTAAAGGCGGT AATTGCAAGCACGATTTGGG 50.0 45.0 58.2 57.9 415-422 bp 1.0 79.7